ASSOSCIATION OF ANAEROBIC BACTERIA IN SUB GINGIVAL PLAQUE AND ORAL HEALTH OF WOMEN

Kanakam Elizabeth Thomas* and Dr. S. Savithri

ABSTRACT

Aim: To determine the prevalence of periodontitis in women, covering comprehensively the entire life cycle of women, namely pre pubertal, pubertal, post pubertal, child bearing age, pregnancy and menopause and to confirm the identity of seven obligate anaerobes with Real Time PCR. 1. Aggregatibacter actinomycetomcomitans 2. Porphyromonas gingivalis 3. Treponema denticola 4. Tannerrella forsythia 5. Capnocytophaga gingivalis 6. Fusobaacterium nucleatum 7. Prevotella nigerescens Material and Method: The periodontal status of each patient was determined with the help of a periodontition by calculating the CAL (Clinical attachment Level) and Probing depth. Sub gingival plaque was collected with the help of Wilsons probe. The plaque sample was subjected to Real Time PCR. Results: It was found that there was an increase in the percentage of periodontitis in women at Puberty, post puberty, pregnancy and at menopause. In our study we found that there is a higher prevalence of Tannerella forsythia in the current study population when compared to the other obligate anaerobes. Discussion and summary: In reports from American academy of Periodontology, it is pointed out that anaerobic bacteria like Porphyromonas gingivalis, Prevotella and Tannerella forsythia are the etiological agents for periodontitis. Similar results were found from studies done by Leonhardt, Jensen et al and Kamma et al,. In our study we found that T.forsythia was more prevalent when compared to the other anaerobic bacteria. The presence of these anaerobic bacteria in the subgingival plaque of our study population suggests the presence of periodontitis among women at puberty, pregnancy and at menopause.

Keywords: Porphyromonas gingivalis, Prevotella and Tannerella.