PRODUCTION AND CHARACTERIZATION OF A POLYCLONAL ANTIBODY AGAINST BUR6 A SUBUNIT OF NC2 COMPLEX INVOLVED IN CANDIDA ALBICANS DRUG RESISTANCES

Vipin Yadav and P. J. John

ABSTRACT

Candida albicans is an opportunistic pathogen having clinical importance as it causing oral, vaginal or systemic infections in immuno-compromized patients, such as HIV+, cancer patients receiving chemotherapy and patients went through organ transplant. Cases of Candida infection dramatically increased due to emergence of drug resistance against antifungal drugs. Previously it was reported that Ncb2 the subunit of NC2 Complex is involved in drug resistance mechanism but the role of Bur6 which is another subunit of NC2 complex is still unknown. To identify the role of Bur6 in Candida drug resistance, complete ORF of Bur6 was cloned and expressed in pET28a vector. Purification of Bur6 protein was performed and polyclonal anti sera against full length Bur6 were raised in Mouse model and further experiments performed to characterize role of Bur6 protein.

Keywords: Candida albicans, drug resistance, transcriptional regulation.