IN VITRO EVALUATION OF BACTERIOPHAGES AGAINST BACTERIAL PATHOGENS FROM WOUND INFECTIONS

Saraswathi R., Prabhusaran N.*, Sherin Beatrice R., Velayutharaj A. and Uma A.

ABSTRACT

The insurgence of antimicrobial resistance is the most common attrition in the management of infectious diseases. In modern medicine, the antibiotics are considered as bedrock in treating drug resistant bacteria. By thinking the alternative, the authors proposed a research by determining in vitro analysis of antimicrobial activity of isolated specific bacteriophages. The main objective of this study is to construct bacterial specific phages from the environmental sources and evaluate in vitro effectiveness against wound infection causing bacterial pathogens. The isolation procedures were optimized and bacteriophages were isolated from raw sewage. After confirming the bacterial pathogens from clinical samples, the phage activity was performed. As a result, plague forming units were identified and counted to confirm the bacterial phage particles. Further, it was identified that the isolated phages were infected the bacterial host cells and no significant observation of the bacterial growth. In conclusion, phage therapy may be an important alternative to antibiotics for treating multidrug resistant pathogens causing superficial infections.

Keywords: Bacteriophages, sewage, plague forming units, antibacterial activity, in vitro.