PROTECTIVE ROLE OF NIGELLA SATIVA OIL IN ISONIAZID INDUCED HEPATOTOXICITY IN RATS

Joydeep Paul*, Mohammad Nasiruddin, Rahat Ali Khan, Saydeeul Hasan Arif

ABSTRACT

Objectives: Hepatotoxicity is one of the major side effects of the first line anti-tubercular drug Isoniazid. Nigella sativa oil is a well researched medicinal plant with reported hepatoprotective activity. The aim of the present study was to evaluate the protective role of Nigella sativa oil in hepatotoxicity induced by using Isoniazid. Methods: Isoniazid (50mg/kg) was used to induce hepatotoxicity in rats. Silymarin (50mg/kg) was used as a standard drug for this study. Silymarin and two doses of N.sativa oil (0.5ml/kg and 1ml/kg) were given for 31 days; isoniazid was started on the 4th day of the study. All drugs were given orally. After 31 days blood samples were collected from the animals for biochemical analysis and liver tissues were subjected to histopathological examination. Results: N.sativa oil significantly reduced the liver enzymes and total bilirubin when compared to the negative control group. There was also significant improvement in the histopathological scores in N. sativa oil treated group when compared to the negative control group. Conclusion: Present study throws light on the usefulness of Nigella sativa oil in hepatotoxicity induced by isoniazid in a dose dependant manner.

Keywords: hepatoprotective, Silymarin, N.sativa.