IN VITRO CYTOTOXICITY OF MARINE SPONGE SPONGIA TOSTA IN A-549, HepG2, HT-29 AND Vero CANCER CELL LINES.

Archana R. and Shabana Begum

ABSTRACT

Cancer is a multigenic disease causing about one fifth of the deaths in each year worldwide. Cancer is caused mainly due to mutation or malfunction of cell cycle controlling machineries. Standard treatment for curing cancer including chemotherapies, coupled with radiation therapies are available but sometime they are associated with severe side effects, as for example, radiation therapies partially disrupts patient’s normal immune systems. Nausea, vomiting, loss of appetite are also common side effects of those standard treatment. Drug development using marine bioresources is limited even though the ocean occupies about 70% of the earth and contains a large number of biological materials. In this study, the silver nano particles synthesized methanolic extract of Marine sponge spongia Tosta (AgNPs -MEMSST) was examined for anticancer activity against lung cancer cell line, colon cancer cell line, liver carcinoma, kidney-African green monkey cell line. The sponge extract dose dependently inhibited viability in this cell line. Our results indicated that methanolic extract of sponge suppressed cell growth in these cell lines, hence the extract possess excellent anticancer potential that may be used for therapeutic purpose of cancer treatment with proper evaluation procedures.

Keywords: Cancer cell line, Anticancer, In vitro, Marine sponge - spongia Tosta.