A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF ARSENIC IN ENVIRONMENTAL AND MEDICINAL LEAVE SAMPLES USING BENZIL-5-AMINO-1,3, 4- THIADIAZOLE-2-THIOL (BZATDT)

Y. Paul Raj, K. Deepa, Y. Lingappa, G. Venkata Reddy, A. Anil Kumar and T. Shobha Rani*

ABSTRACT

Benzil-5-amino-1,3,4-thiadiazole-2-thiol (BZATDT) has been proposed as new analytical reagent for the direct non-extractive spectrophotometric determination of Arsenic. The reagent reacts with arsenic in acidic medium (pH 4.0, Sodium acetate- acetic acid buffer) to form light yellow colored 1:1 (M:L). The colour reactions are instaneous and the maximum absorbance was obtained at 346 nm and remains constant for over 4 h. The molar absorptivity and Sandell’s sensitivity of BZATDT found to be 5.70 x 103 L mol-1cm-1 and 0.00175 μgcm-2 of AsIII respectively. The system obeys Beer’s law in the range of 0.126 - 2.64 μg/ml of AsIII. Since BZATDT method is more sensitive it was applied for determination of Arsenic in some environmental water, soil and medicinal leave samples.

Keywords: Spectrophotometry, Arsenic determination, BZATDT, Environmental and medicinal leave samples.