ANTI-INFLAMMATORY EFFECT, ANTIOXIDANT POTENTIALS AND PHYTOCHEMICAL INVESTIGATION OF CRATAEGUS SINAICA BOISS. ROOTS GROWING IN EGYPT.

Atef A. El-Hela, *, Nevein M. Abdelhady, Hala M. El-Hefnawy, Taghreed A. Ibrahim and Gamil M. Abdallah

ABSTRACT

In vivo antioxidant and anti-inflammatory studies of the aqueous and methanol extracts, as well as, methylene chloride and ethyl acetate fractions of Crataegus sinaica Boiss. roots (Rosaceae) growing in Egypt were carried out. Anti-inflammatory effect was evaluated in rats using paw oedema ,which revealed that the methylene chloride fraction was the most potent followed by methanol extract, ethyl acetate fraction and aqueous extract respectively, meanwhile, the antioxidant potentials was performed through evaluation of their impact against Streptozotocin induced oxidative stress in liver and kidney tissues of albino rats revealed that the ethyl acetate fraction was the most potent followed by methanol and aqueous extracts, then methylene chloride fraction respectively. Phytochemical investigation of the methylene chloride and ethyl acetate fractions exhibiting the most significant anti-inflammatory and antioxidant effects resulted in isolation of four compounds, namely, cholesterol, ß-sitosterol, uvaol and oleanolic acid, and five compounds, namely, epicatechin, quercetin, hyperoside, rutin and vitexin respectively, the structures of the isolated compounds were elucidated on basis of their chromatographic and spectroscopic data.

Keywords: Crataegus sinaica Boiss., Egypt, Roots, Triterpenes, Flavonoids, Anti-inflammatory and Antioxidant.