EXTRACTION AND CHARACTERIZATION OF BIOFILM PRODUCED BY VS3D STRAIN AND ITS DYE ADSORPTION EFFICIENCY

P. Srinivasan, P. Thiyagarajan, A. Kalirajan, A. Sengottaiyan, C. Sudhakar and T. Selvankumar*

ABSTRACT

In the present investigation, four soil samples were collected from various locations of erode district. The soil samples were subjected to serial dilution technique which resulted in the isolation morphologically distinguishable five different bacterial strains designated as BS3B, BS1F, BS1F, VS3D, and VS3A. The five bacterial strains were screened for its biofilm production activity using tube ring assay and confirmed as best biofilm producers. The isolates were subjected to biofilm production using LB broth medium supplemented with 0.3 % chitin flakes. The formation of biofilm was confirmed by SEM imaging which showed the presence of biofilm cells on chitin flakes. Bio adsorption of dyes by the biofilm produced by the above isolated were performed by the absorption spectrum of visible spectrophotometer read at 528 nm. Based on the spectral data VS3D strain was found with potential dye adsorbing activity. The potential strain VS3D was tested for its dye adsorbing activity using different concentration of extracts (50, 100, 200, and 300 μg/ml ).The results showed dye adsorbent potential of biofilm produced by the bacterial isolated VS3D. The biofilm produced by the strain adsorbed 50μg/ml concentration of dye when observed after 120 hrs of incubation time.

Keywords: Bacterial strains, biofilm, tube assay, textile dye, SEM, decolorization.