DETERMINATION OF PERMEATION PATHWAY OF TETRACYCLINE INTO THE PIG’S EAR SKIN

Fadli A.*, Mohamad Nizam A. G., Ibrahim A., Shariatul Nabila M. S., Jiyauddin K., Samer A. D., S. Budiasih, M. Kaleemullah, Jawad A., Rasha S., Todo H., Sugibayashi K. and Eddy Y.

ABSTRACT

The important roles of hair follicles as permeation pathway of topically applied drugs have gained recent attentions in several fields of research especially dermatological sciences. Hair follicles are increasingly considered to represent as one of drug delivery routes through the stratum corneum into deeper skin layers. This study was conducted to confirm the penetration pathway of Tetracycline into pig’s ear skin through comparing the concentration of Tetracycline between nonplugged pig’s ear skin and plugged pig’s ear skin which were done through hair follicular blocking method by using silicone. The findings from this study showed that the permeation of tetracycline through non-plugged pig’s ear skin is higher compared to plugged pig’s ear skin. As such, the maximum concentration of tetracycline permeated through plugged pig’s ear skin was generally lower than non-plugged pig’s ear skin. Statistical analysis by using ttest showed that P-value from the cumulative amount of permeation of Tetracycline in both non-plugged and plugged pig’s ear skin is P<0.05. Thus, the difference between cumulative amount of concentration of Tetracycline for duration of 24 hours study between non-plugged and plugged pig’s ear skin are considered to be statistically significant as P<0.05. Thus, the present findings clearly suggest that the permeation pathway of tetracycline drug into pig’s ear skin is through hair follicles.

Keywords: Tetracycline, Hair follicles, Pig’s ear skin, Permeation pathway.