IN VIVO ASSESSMENT OF IMMUNOMODULATORY ACTIVITY OF WITHANIA COAGULANS DUNAL FRUITS

Archana K. Shendkar*, Sugandha G. Chaudhari, Usama Khan and Dr. Yogesh K. Shendkar

ABSTRACT

The aim of the study was to investigate the immunomodulatory activity using methanolic and hydro-alcoholic extracts of Withania Coagulans dunal fruits i.e. Withania Coagulans Methanolic Extract (WCME) and Withania Coagulans Hydroalcoholic Extract (WCHAE). Phytochemical analysis has indicated presence of alkaloids, flavonoids, glycosides, steroids, saponins, fixed oil, carbohydrates. Both the extracts have showed immunomodulatory activity by Serum hemagglutinin titer assay. The agglutination reaction between test serum antibodies and antigen (mesh formation) present in Sheep Red Blood Cell’s effectively inhibited by 75%, 77.77% in standard (Methotrexate), 40%, 44.44% in WCME-100 mg/kg, 55.55%, 60% in WCME-200 mg/kg, 50%, 66.66% in WCHAE-100 mg/kg, 70%, 75% in WCHAE-200 mg/kg groups in comparison with agglutination of control group wells in micro-titer plates. Both the extracts showed the good immunomodulatory activity but WCHAE showed the most significant immunomodulatory activity than WCME. The findings of the present study suggested that Withania Coagulans dunal fruits could be a potential natural source of immunomodulatory activity which is very important against treatment of immune system disease.

Keywords: immunomodulatory, Withania Coagulans, Setumhemagglutinin titer, agglutination.