IN VITRO CULTURE AND EVALUATION SOME PHYTOCHEMICAL COMPOUNDS OF ERIOCEPHALUS AFRICANUS L. PLANT.

Tarek Abou Dahab Mohamed, Afaf Mahmoud EL Sayed Habib, Mohamed Mahmoud ELzefzafy, Gouda T. M. Dawoud* and Ayman I. EL- D. Soliman

ABSTRACT

The effect of some factors on growth and development of Eriocephalus africanus L. plant in vitro, showing that B5 basal medium gave the best results in most of growth measurements when culturing the shoot tips explants. 2.0 mg/l BAP recorded the highest values in survival percentage, shootlets number /cluster and shooter strength 93.33%, 16.50 and 4.50, respectively. Use the high level from GA3 4.0 mg/l in medium was more effect in the elongation of shootlets. In rooting stage the moderate concentration from IBA 0.5 mg/l was more effect at increased roots number/explant and root length to 8.67 and 5.78cm, respectively. Planting media containing Peatmoss, Sand and Vermiculite interaction at equal volume 1:1:1 v/v was recorded the best results in acclimatization of rooted plants. The phenolic composition from plants of E. africanus L. in vitro produced and in vivo grown was qualitatively and quantitatively analyzed by using High Performance Liquid Chromatography HPLC which identified compounds namely Gallic acid, Chlorogenic acid, Caffeic acid, Catechin, Quercetin and Kaempferol from E. africanus. The antioxidant activity by using DDPH Free radical scavenging were investigated in vitro produced plants and in vivo grown plants at outside the greenhouse (mother plants) the obtained 81.01% and 78.58 mg %, respectively.

Keywords: Eriocephalus africanus, in vitro and in vivo, Acclimatization, antioxidant activity, Phenolic and Flavonoids and HPLC.