FT-IR ANALYSIS AND IN VITRO ANTIBACTERIAL ACTIVITY OF PROSOPIS JULIFLORA

Nagalakshmi G. and Anuradha R*.

ABSTRACT

Prosopis juliflora, an Indian Mallow has been used in the treatment of various diseases in different parts of the world since time immemorial. The present investigation was carried out to find the effect of methanolic extracts of flowers of Prosopis juliflora through FT-IR Spectroscopy method and its antibacterial activity. The FT-IR analysis confirmed the presence of phenol, alcohols, alkanes, carboxylic acids, ketones, alkenes, aromatics, ethers, and aliphatic amines compounds, which showed major peaks. The anti bacterial activity of the methanolic extracts of Prosopis juliflora (25,50,75,100, 200,300mg/ml) was also tested against two human pathogenic bacteria such as Escherichia coli and Staphylococcus aureus. The anti bacterial potent of the extracts was found to be dose dependent. The phytochemical analysis of the plant was carried out. The results revealed the quantitative analysis of following classes of natural constituents: flavonoids, alkaloids, saponins, phenols and tannins in the range of (25%), (15%), (10%), (22%) and (18%), respectively. The crude fiber and ash content were also analyzed and found as (12%) and (7%), respectively whereas, pectic substances were calculated as (12%). The activity of the flower of Prosopis juliflora was due to the presence of various secondary metabolites. Hence the plant can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.

Keywords: Prosopis juliflora, FT-IR analysis, in vitro antibacterial activity.