STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF MUPIROCIN AND BECLOMETHASONE DIPROPIONATE IN PHARMACEUTICAL DOSAGE FORM

Prasanna Pradhan*, Dhanga Nehal, Chaudhari Sandhya, Shreya Shah and Umesh Upadhyay

ABSTRACT

A simple, rapid, economical, precise and accurate Stability indicating RP-HPLC method for simultaneous estimation of Mupirocin and Beclomethasone dipropionate in Their Combined Dosage Form has been developed. The separation was achieved by LC- 20 AT C18 (250mm x 4.6 mm x 2.6 μm) column and Buffer (pH 4.5): Acetonitrile (60:40 v/v) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 236 nm. Retention time of Mupirocin and Beclomethasone dipropionate were found to be 3.55 min and 5.18 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Mupirocin 20-60 μg/ml and for Beclomethasone dipropionate 0.5-1.5 μg/ml. The percentage recoveries obtained for Mupirocin and Beclomethasone dipropionate were found to be in range of 100.22 ± 0.60 and 100.59± 0.20 respectively. The proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial combined dosage form.

Keywords: Mupirocin, Beclomethasone dipropionate, Stability indicating RP-HPLC Method, Validation.