COMPARISON OF LOCAL ANTI-INFLAMMATIORY POTENCY OF CLOBETASOL, MOMETASONE AND HYDROCORTISONE IN GUINEA PIGS

Rajeshwari Gore, *Kamlesh Garg, Yashika Garg, Piyush Setu, Shalini Gore, Jayaprabha Mane-Gambhire

ABSTRACT

Objectives: To compare the anti-inflammatory potency of three topical corticosteroids Clobetasol, Mometasone and Hydrocortisone by histamine induced wheal suppression test in guinea pigs. Material and Methods: Adult male guinea pigs were placed in 4 groups with 6 animals in each group. Each animal received one of the three test drugs, i.e. Hydrocortisone, Mometasone or Clobetasol and one group was kept as a control. The back of the guinea pig was shaved and histamine was injected intradermally to produce a wheal and flare reaction. Dose and time required for suppression of the wheal was noted in each group and flared was graded by Visual Analogue Scale. One way analysis of variance (ANOVA) test was applied to compare the time taken for wheal suppression by the test groups, P-value 0.05 was considered significant. Results: Earliest suppression of the wheal was shown by Clobetasol group with minimal dose (0.05%), followed by Mometasone (0.1%) and Hydrocortisone with maximum dose (1%). The flare produced in clobetasol group was mild, while that in Mometasone was moderate and Hydrocortisone was severe. Conclusion: Clobetasol belonging to the most potent group showed earliest and complete suppression of wheal and flare reaction with minimal dose followed by Mometasone having with intermediate potency while Hydrocortisone which was least potent did not show complete suppression of the wheal till last observation even with maximum dose.

Keywords: Topical corticosteroids, Clobetasol, Mometasone, Hydrocortisone