ORAL ?- TOCOPHEROL SUPPLEMENTATION AMELIORATES THE SERUM MALONDIALDEHYDE, SOD, GPX, CAT, GSSH, BLOOD LIPIDS AND GLYCEMIC CONTROL IN TYPE 2 DIABETIC SUBJECTS

Dr. Suhail Ahmed Almani, Dr. Ali Naseer, Dr. Shahzore Gul, Dr. Sanjay Kumar Maheshwari, Dr. Raza Naseer and *Dr. Arsalan Ahmed Uqaili

ABSTRACT

Background: Vitamin E (α- tocopherol) is a natural anti oxidant vitamin found in large quantities in blood and cell membrane. The α-tocopherol exerts anti oxidant and anti lipid peroxidant activity and influences the insulin sensitivity. Objective: To analyze the effects of Oral α- tocopherol supplementation on the serum malondialdehyde, SOD, GPX, CAT, GSSH, blood lipids and glycemic control in type 2 Diabetic (T2DM) subjects. Methods: The present observational study was conducted at the Department of Medicine, LUMHS Jamshoro/Hyderabad from January 2014 to March 2015. 50 diagnosed cases of type 2 DM were labeled as controls and 50 type 2 DM+ oral α- tocopherol 400 mg/day as cases. At the end of three months, blood samples were centrifuged at 4000 rpm and sera were stored at -200C. HOMA-IR was calculate by formula; fasting insulin × fasting glucose/22.5. Blood glucose, HbA1c, fasting insulin, serum creatinine and serum bilirubin were estimated. Assay kits were used for the estimation of SOD, GPX, CAT, GSSH and MDA. Data was analyzed on Statistix 8.1 (USA) at 95% confidence interval (P ≤ 0.05). Results: Malondialdehyde (MDA) was reduced in T2DM subjects taking Oral α- tocopherol; cases 4.78±2.85 vs. controls 6.08±2.16 μmol respectively (p=0.012). Systemic blood pressure, blood glucose, blood lipids, serum insulin, HOMA-IR, serum SOD, GPX, CAT, GSSH and serum bilirubin were improved T2DM supplemented with oral α- tocopherol compared to controls. Conclusion: Oral α- tocopherol supplementation for 3 months exerted anti-peroxidant and anti oxidant effects significantly with improved glycemic status and blood lipids.

Keywords: Oral ?- tocopherol Malondialdehyde Antioxidant enzymes Glycemic control Diabetes mellitus.