EFFECT OF CARBON AND NITROGEN SOURCEAND MEDIA OPTIMIZATION FOR THE PRODUCTION OF GLUCOSE ISOMERASE FROM ARTHROBACTER SPECIES (5327)

Jayaprabha Chockalingam*, Swathy Krishna Jayalekshmi, Arya R. Krishna

ABSTRACT

The present study is to isolate and screening of glucose isomerase from arthrobacter species (5327) for industrial application of fructose. Arthrobacter has emerged as an in exhaustive treasure for a wide range of enzymes like glucose isomerase. Glucose isomerase screening was performed by three different methods such as plate assay using xylose containing peptone yeast agar medium, protein estimation by barfods test. Arthrobacter species (5327) were grown on peptone yeast extract broth, the cell biomass was harvested then disrupted by ultrasonic disintegrator and centrifuged; cell free extracts were used for glucose isomerase activity. Fixing of carbon and nitrogen source in a minimum concentration was assayed and the shake flask experiments are done which has proved numerous Arthrobacter species (5327) has been produced glucose isomerase activity, particularly it has been proved superior performing for glucose isomerase production and can be used for industrial application of fructose production.

Keywords: Arthrobacter Species, Glucose isomerase, Enzyme assay.