BIOSYNTHESIS OF SILVER NANOPARTICLES FROM SEED POWDER EXTRACTS OF LATHYRUS SATIVUS, MEDICINALLY POTENT LEGUME PLANT

Veera Babu Nagati, Swathi Tenugu, Rama Koyyati and Pratap Rudra Manthurpadigya*

ABSTRACT

Biological synthesis of nanoparticles has been an exploring research topic in recent days due to their advanced use in biomedical, chemical and related fields. Bioreduction of silver nitrate (AgNO3) with the help of plant extract is a great advantage in green synthesis of nanoparticles. In the present investigation, Silver Nano-particles were rapidly synthesized using seed extract of Lathyrus sativus, a member of Leguminaceae family. Seed extract was mixed with 1mM silver nitrate and incubated at 60°C for 15 min. The reddish brown color confirms the formation of Lathyrus sativus silver nanoparticles (LsAgNPs). The formed silver nanoparticles were then characterized by UV-Visible spectrum, FTIR-spectrometry. SEM analysis and TEM analysis revealed that the morphology and size of the synthesized silver nanoparticles were in the range of 10-40 nm. These biologically synthesized nanoparticles also showed inhibitory effects on Pseudomonas putida and Staphylococcus aureus. The plant materials mediated synthesis of silver nanoparticles is comparatively rapid; eco-friendly; less expensive and has wide applications like antibacterial therapy in modern medicine.

Keywords: Lathyrus sativus, ODAP, Homoarginine, Transmission electron microscope, Bioreduction.