CROTON MACROSTACHYUS ROOTS USED TO STUDY PHYTOCHEMICAL SCREENING, TEST FOR INORGANIC ELEMENTS, PROXIMATE ANALYSIS, QUALITATIVE AND QUANTITATIVE CHARACTERIZATION OF PHYTOCONSTITUENTS, ANTIOXIDANT ACTIVITIES AND FREE RADICAL SCAVENGING ACTIVITY

Kiran Kumar S. J.* and Deenadayalan K.

ABSTRACT

The present study was to evaluate preliminary photochemical analysis, inorganic elements, antioxidant activities, proximate analysis and free radical scavenging activity of root extracts of Croton macrostachyus by using different solvent like methanol, petroleum ether, ethyl acetate and chloroform. Phytochemical analysis showed the presence carbohydrate, reducing sugars, phenolics, flavonoids, glycosides, saponnins and steroids. The moisture content of the crude sample was found to be 9.01 ± 1.20 while the water extractive index was 1.14 ± 0.036. Quantitative analysis of phenols, flavonoids and Triterpenoids was further performed. Preliminary phytochemical screening of roots were by methanolic extracts which identified 12 major functional groups, amongst them flavonoid, phytosterols, saponins and coumarins. Additionally inorganic elements like iron, chloride and sulphate were identified by total ash analysis. The same extract was used for quantitative determination of total flavonoid content (81.88 mg/g), total phenolic content (67.78 mg/g) and total triterpenoids content (13.22mg/g). The methanolic extract had the highest antioxidant property compared to other fractions.

Keywords: Croton macrostachyus, phytochemical screening, proximate analysis, antioxidant activity, proximate analysis, free radical scavenging activity.