DEVELOPMENT AND VALIDATION OF AREA UNDER CURVE AND FIRST ORDER DERIVATIVE UV SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF BENFOTIAMINE IN BULK AND SOLID DOSAGE FORM

Niraimathi V.* and Siva Sankari K.A.

ABSTRACT

Simple, sensitive, efficient and economical spectrophotometric methods have been developed for the estimation of benfotiamine in bulk as well as in tablet dosage form. The first order derivative spectrophotometry method and area under curve method obeys Beer’s law concentration in the range of 4-20μg/mL. The overall percentage purity for the sample by both the methods was found to be between 99-100% and the percentage recovery was found to be 99-101% which indicates the accuracy of the proposed method. The methods have been validated as per ICH guidelines and could be applied in future analysis of Benfotiamine formulations. Both the methods showed good reproducibility and recovery. The results of the methods were validated statistically and found to be satisfactory.

Keywords: Simple, economical first order derivative method, area under curve method.