VALIDATED SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF NITROFUTOIN IN PURE AND IN ITS DOSAGE FORM

P. Suguna*, B. Sathyanarayana, G. Chandrasekar and B. Narasimhulu

ABSTRACT

A simple, précis, rapid sensitive and accurate spectrophotometric methods have been developed for the estimation of Nitrofuratoin UV in pure form and its pharmaceutical formulations based on oxidative coupling reaction UV with MBTH reagent at PH - 4 which is extractable at 620 nm. Beer’s law is obeyed in the concentration range 1- 6 ml (10-60 μgml-1) .The developed method was applied directly and easily for the analysis of the pharmaceutical formulations. RSD was found to be 0.0194% and recovery 99.73%. The method was completely validated and proven to be rugged. The interferences of the ingredients and excipients were not observed. The repeatability and the performance of the proved method were established by point and internal hypothesis and through recovery studies.

Keywords: Spectrophotometry, Nitrofuratoin, MBTH, Oxidative coupling.