PREPARATION, SYNTHESIS AND CHARACTERISATION OF SILVER NANOPARTICLES BY FISH SCALES OF CATLA CATLA AND THEIR ANTIBACTERIAL ACTIVITY AGAINST FISH PATHOGEN, AEROMONAS VERONII

D. Vineela, S. Janardana Reddy* and B. Kiran Kumar

ABSTRACT

Silver has been in use since time immemorial in the form of metallic silver, silver nitrate, silver sulfadiazine for the treatment of burns, wounds and several bacterial infections. But due to the emergence of several antibiotics the use of these silver compounds has been declined remarkably. Nanotechnology is gaining tremendous impacts in the present century due to its capability of modulating metals in to their nanosize, which drastically changes the chemical, physical and optical properties of metals. From the results, unveil that the synthesized silver nanoparticles were characterized by SEM, UV-VIS, and FT-IR spectroscopy. In the present work successfully synthesized AgNPs from the fish scales of Catla catla which are biocomposites of high ordered collagen fibres, hydroxyapatite and amino acids. AgNPs synthesis was confirmed by colour change, wave range of UV-VIS, FT-IR spectroscopy and the shape and size of the nanoparticles has made a remarkable comeback as potential antimicrobial agents. The synthesized silver nanoparticles were used to study the Antibacterial activity against the fish pathogen A.veronii by the application of 100μl of 1 M silver nanoparticle. Silver nanoparticles show greatest potentiality towards controlling the growth of bacteria.

Keywords: Silver nanoparticles, Aeromonas veronii, Catla catla, Fish Scales, Synthesis, SEM analysis, UV-VIS, FT-IR, Antibacterial activity, Zone of Inhibition.