IN VITRO ELIMINATION OF ARSENIC FROM WATER THROUGH LACTOBACILLUS SPOROGENES

Ranjit Kumar*, ShaliniKumari, PratibhaKumari, Arun Kumar, Mohammad Ali, Pintoo Kumar Niraj and Ashok Ghosh

ABSTRACT

The problem of the occurrence of arsenic in groundwater has gained worldwide attention and studies in high-exposure regions of the world have established health issues including skin lesions, melanosis and arsenicosis. Probiotics are defined as live microbial food ingredients that have a beneficial effect on human health. Lactobacillus sporogenes is a gram-positive, spore-forming, lactic-acid producing bacillus. It is used in prevention and cure of viral, bacterial and antibiotic or radiotherapy induced diarrheas, immunomodulation and even blood cholesterol reduction. The present study is designed to understand effect of Lactobacillus sporogenes on In-Vitro elimination of arsenic from water. Arsenic samples of 100 ppb, 200 ppb and 500 ppb is freshly prepared and 50,000 spores were introduced into each culture in one arm study in second arm 50,000 spores were introduced into these samples with nutrient agar as culture medium. It is observed that lactobacillus without culture media eliminate arsenic effectively only in 24 hours while lactobacillus with nutrient agar media causes more effective elimination of arsenic from water sample in 48 hours. It is concluded from study that Lactobacillus sporogenes causes effective In Vitro elimination of arsenic from water. When Lactobacillus is introduced with Nutrient agar culture is eliminate arsenic more effectively indicates that whenever Lactobacillus sporogenes grows properly arsenic adhere with its growing cell wall and eliminated with the bacterial colony. Lactobacillus sporogenes removes more than 90% of arsenic from water samples effectively.

Keywords: In Vitro, Lactobacillus sporogenes, bacteria, Diarrhea, Spores.