THE ACTION OF SOFT LASER ON SUBMANDIBULAR SALIVARY GLAND OF MICE

*Aedah Z. Al-Kaisy

ABSTRACT

The aim of this study was to calculate the histological changes on the salivary gland in diseased mice that inoculated with inoculated with Candeda in the oral cavity, twenty four white mice were divided into three groups each of six mice and the six remain taken as control. The mice were sacrificed after 15 days of continuous irradiated with soft type Gallium –Arsenide of 812 nm wave length and power 100wa. The gland were prepared in paraffin and analyzed with light microscop. the result showed astatically significant increasing of the secretion of the gland acini of the mice when it compared with the control group that not radiated. The diameter of the gland was increased from 2.01mm to 3.02mm for group A that irradiated with time interval 2hr for twice daily and be 2.3mm for group that irradiated three time daily, at the end of the experiment (20) days control and at the experimental mice were sacrificed by either application the left submandibular gland was carefully dissected intact ,for histological studies; the glands were placed in 10% buffered formalin and processed by conventional methods for embedded in paraffin sections were obtained and eosin (H&E) for light microscopy evaluation. The diameter of acini and other structure of both control and experimental groups were measured by using calibrated ocular lens, for each case there are two observation measurements of the diameter of acini then we take the mean of these two measurements. The same procedure were applied for the control, then statistical analysis significance was evaluated by t-test.

Keywords: w Level Laser, in medicine, Salivary gland, Laser for treatment.