QUANTITATIVE ESTIMATION AND VALIDATION OF ATENOLOL AND AMLODIPINE BESYLATE BY ABSORBANCE RATIO (Q) METHOD.

Rahul K. Godge*, Ganesh S. Shinde and Nachiket S. Dighe

ABSTRACT

The simple, rapid, accurate, precise, cost effective, and reproducible UV spectroscopic method have been developed for the simultaneous estimation of atenolol and amlodipine besylate in bulk and combined tablet dosage form. Atenolol and amlodipine have absorption maxima at 224 and 238.2 nm respectively. Beer’s law obeyed in concentration range of 2‐24 μg/ml and 2‐34 μg/ml for Atenolol (ATN) and Amlodipin (AMN) respectively. The method of Q analysis is based on measurement of absorptivity at 224 nm and at isoabsorptive point 232.2 nm. The recovery studies from tablet are indicative of accuracy of method and are found in between 99.05‐101.16% at three different levels of standard additions. Precision studies showed satisfactory results. A novel approach to use 0.1N HCL as solvent is proved to be beneficial with respect to cost, stability and avoidance of organic solvent.

Keywords: Atenolol, Amlodipine Besylate, 0.1 N HCL, Q Method.