ISOLATION AND CHARACTERIZATION OF ANTI-HYPERGLYCEMIC COMPOUND FROM FRACTIONATION OF ETHANOLIC EXTRACT OF KALANCHOE PINNATA LEAF.

Dr. Pradeep Goyal*, Dr. Jitender Singh, Nishant Thakur and Dr. Manish Goswami

ABSTRACT

The present study was aimed to evaluate the effect of active compounds from Kalachoe pinnata leaves on blood glucose level in normal and diabetic rats. Diabetes was induced by Streptozotocin (STZ) in male wistar rats. An isolated fraction of EEKP (600 mg/kg b. wt.) and Glibenclamide (10 mg/kg b.wt.) was administered orally. An isolated fractions showing for higher antihyperglycemic activity was subjected to column chromatography that led to isolation of a pure compound, which was given trivial name KP-1. The fraction H from EEKP was found to lower the blood glucose level significantly (P<0.05) in diabetic rats. To ensure the compounds responsible for anti-hyperglycemic activities associated with H respectively. Further column chromatographic analysis was carried out using various solvent systems and which was given trivial name KP-1. KP-1 was phenolic compound nature (Flavonoids) confirmed by spectral analysis.

Keywords: K.pinnata, Antihypergycemic, Flavanoid.