SYNTHESIS, CHARACTERIZATION AND CYTOTOXIC EVALUATION OF 5-[4-(1HIMIDAZOL- 1-YL)PHENYL]-3-(2,4,6-TRIMETHYLPHENYL)-4,5-DIHYDRO-1,2- OXAZOLE DERIVATIVES BY USING MTT ASSAY METHOD

Gondu Eswara Rao*, S. A. Rahaman and A. Prameela Rani

ABSTRACT

The isoxazoline derivatives were synthesized from chalcone as intermediate compound. the chalcones were reacted with hydroxyl amine in presence of glacial acetic acid and sodium acetate to form cyclic compound isoxazoline derivatives and the structures were confirmed by spectral evidence. The compounds were tested for cytotoxic evolution by MTT assay method. . In these test compounds, The compound IS-02, (45-[4-(1H-imidazol-1-yl)phenyl]-3-(3,4,5-trimethyl phenyl)- 4,5-dihydro-1,2-oxazole 15±2 μg/ml, 18±1 μg/ml,12±1 μg/ml against the Breast cancer (MDA MB, Colon cancer (HT-29), Prostate cancer(DU-145)

Keywords: Isoxazoline derivatives, urea, cytotoxicity, MTT assay method.