MARKER BASED HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD DEVELOPMENT AND VALIDATION FOR ANALYSIS OF SEED OIL FROM ANNONA SQUAMOSA

Sandeepa Suresh Bhoir* and K. K. Singh

ABSTRACT

Herbal medicines are widely used for disease treatment, even when their biologically active compounds are unknown. Although Annona squamosa (Custard apple) has high medicinal value, the precise methods to study the active components are not investigated. A simple, precise and accurate marker based high performance liquid chromatographic technique has been developed for quantitative estimation of linoleic acid from Annona squamosa oily seeds (ASO) extract. The chromatographic separation was performed on C-18 column Qualisil (150mm x 4.6mm, 5μm) as a stationary phase and mobile phase comprising of Acetonitrile: Methanol: THF: water containing 0.5% glacial acetic acid (36:32:31:1 v/v) at a flow rate of 0.5ml/minutes and analysis was screened with UV detector at 236nm. The proposed method was linear within the concentration range of 4-100 μg/ml as well as accurate, precise and specific. Thus validated marker based HPLC method can be used for a routine quality control analysis and quantitation of linoleic acid from oil extracted from Annona squamosa seeds and its formulations.

Keywords: Annona squamosa Seed oil, marker based HPLC analysis, Linoleic acid, validation.