ANTI-OXIDANT AND ANTI-BUTYRYLCHOLINESTERASE ACTIVITY OF AN ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA.

Suresh Kumar* and Swati Sharma

ABSTRACT

Cholinesterase inhibitors are the most sought after drugs for the symptomatic treatment of Alzheimer‟s disease. The present study, assessed the anti-oxidant and anti-butyrylcholinesterase property of an ethanolic extract of Tinospora cordifolia’s stem, which is an Ayurvedic plant traditionally used for its several medicinal properties. Our findings demonstrated that an ethanolic extract of Tinospora cordifolia’s stem significantly inhibited butyrylcholinesterase enzyme and showed maximum inhibition of 73.59±0.02% at 200μg/ml final concentration with IC50 value of 37.41 μg/mL calculated from the percentage inhibition curve. The mechanism of enzyme inhibition demonstrated by Lineweaver-Burk plot showed competitive inhibition at higher concentration and mixed non-competitive mode of inhibition at lower concentration of plant extract. Antioxidant activity of the extract was assessed by Trolox equivalent antioxidant capacity assay and Ferric reducing ability of plasma assay, which suggested that this plant also possesses significant antioxidant property. The maximum ferrous sulphate equivalent and trolox equivalent values were found to be 1785.66 ± 0.060 μmol Fe2+ E/g of dried sample and 0.72 ± 0.003 mmol Trolox E/g of dried sample respectively. It is concluded that this plant has potential in deriving lead compound for anti-butyrylcholinesterase and antioxidant property which might be beneficial in future for providing symptomatic relief for AD patients.

Keywords: Alzheimer?s disease, Butyrylcholinesterase, Tinospora cordifolia.