ISOLATION, PURIFICATION AND CHARACTERIZATION OF BIO PESTICIDE (TRICHODERMA) FROM BILASPUR DISTRICT CHHATTISGARH

Aakanksha Sharma, Dr. Shweta Sao* and Dr. Prashant Kumar Sharma

ABSTRACT

Trichoderma spp. is one of the important biocontrol agents, as it grow well at all temperatures and pH, and also has higher antagonistic activity disease controlling potential caused by soil born pathogen. On the basis of temperatures (150C, 250C, 350C and 450C) on Mycelial growth of Trichoderma species was varied growth of all 28 isolates was faster at 250C whereas at low temperature 150C Trichoderma isolate no. T-14 grow at faster compared to other isolate. Isolate no. T-14, T-25, T-32 and T-37 are faster growth 250C, isolate no T-05, T-14, T-25 and T-47 faster growth at 350C and isolate no T-14, T-31, T-25 and T-43 faster growth at 450C can be successfully used bio-control conditions. different pH range influence of different pH (5.0, 6.0, 7.0 and 8.0) on Mycelial growth of Trichoderma species isolate no T-13, T-14 and T-41 was faster growth of pH-5.0 and isolate no T-14, T-32, T-39, T-42 and T-45 was fast growth of pH-6.0 and isolate no T-13 and T-41 are faster growth of pH-7.0 Trichoderma isolate no T-15 and T-31 is faster growth of pH-8.0 comparison than other isolates.

Keywords: Bio Pesticide, Trichoderma, Antagonistic Activity.