CLINICOPATHOLOGICAL CORRELATION OF NON-HODGKIN LYMPHOMA AN IMMUNOHISTOCHEMICAL PROFILE

Nafisa Awadh Mansoor* and Sultan Al-Kubati

ABSTRACT

Introduction: Non-Hodgkin lymphomas (NHL) are assorted group of malignant lymphoproliferative disorder and showing distinctive patterns of behavior and responses to treatment. Objectives: The study aims to assess the diagnostic role of immunohistochemistry for immunophenotyping of non-Hodgkin lymphoma into B-cell and T –cell phenotypes, and to correlate them with age, sex and site of biopsy. Methods: A total of 40 cases of NHL were collected during two years 2012-2013.H&E, IHC were done. The markers used in this study were LCA, CD20, CD3, Ki67, CD10, CD15, CD30, EMA, Bcl2, TdT, CD5, CD34, using En Vision system. Results: 22 cases were males (55%) and 18 cases were females (45%), M: F ratio 1.2:1, age range 2-85. B-cell lymphomas were (87.5%), T-cell lymphomas were (7.5%), unclassified (5%). The most common type of NHL B-cell type was DLBCL (32.5%), followed by Burkitt lymphoma (12.5%), follicular lymphoma (7.5%), MZL (7.5%), B-cell lymphoma unclassified (5%). T-cell lymphomas were three, Precursor T- lymphoblastic lymphoma, T-cell lymphoma (unspecified), anaplastic large cell lymphoma. Two cases were unclassified. The major affected age group was 45-59 (37.5%). Nodal and extranodal were equally affected 50% each. The most common nodal site were cervical lymph nodes (20%). The most common extranodal site were the GIT (17.5%). Conclusions: We concluded that IHC is a very important investigation for all lymphomas and for immunotherapy. IHC is also important for differential diagnosis with other malignancies. B-cell lymphomas outnumber T-cell lymphomas, the most common type of B-cell NHL was diffuse large B-cell lymphoma (DLBCL), followed by Burkitt lymphoma. B-cell lymphoma most commonly nodal with cervical predominant. All T-cell lymphomas were extranodal.

Keywords: Non-Hodgkin lymphoma, immunophenotyping, Yemen.