COMPARATIVE ANALYSIS OF ANTIMICROBIAL ACTIVITY OF LEAF EXTRACTS FROM THREE SPECIES OF GENUS HYDROCOTYLE ON PATHOGENS

Seok Hyeon Moon, Jong Hwa Yum and Man Kyu Huh*

ABSTRACT

Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. The The The The antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts antimicrobial activity of the extracts three species of genus Hydrocotyle was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion was analyzed by using well diffusion method paper disk diffusion amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method a method paper disk diffusion amethod paper disk diffusion method amethod paper disk diffusion method a method paper disk diffusion a method paper disk diffusion a method paper disk diffusion amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method a method paper disk diffusion amethod paper disk diffusion method amethod paper disk diffusion method a method paper disk diffusion amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method amethod paper disk diffusion method a method paper disk diffusion and minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of nd minimum inhibitory concentration. In ethanoic extract of H. ramifloraH. ramifloraH. ramifloraH. ramifloraH. ramiflora H. ramifloraH. ramifloraH. ramifloraH. ramiflora H. ramiflora, maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in maximum inhibition zone diameter among all the tested microorganisms was obtained in Enterobacter nterobacternterobacternterobacter nterobacternterobacternterobacter cloaceaecloaceaecloaceae cloaceaecloaceaecloaceaecloaceae with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. with diameter of 6.1±0.5 mm. H. ramifloraH. ramifloraH. ramifloraH. ramifloraH. ramiflora H. ramifloraH. ramifloraH. ramifloraH. ramiflora H. ramiflora showed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wi showed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wi showed maximum inhibition zone wishowed maximum inhibition zone wi showed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wi showed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wi showed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone wishowed maximum inhibition zone with diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in th diameter of 6.3±0.3 mm in Escherichiascherichiascherichiascherichiascherichiascherichiascherichiascherichiascherichiascherichia coli, coli, coli, coli, coli, coli, 6.1±0.3 mm in 6.1±0.3 mm in 6.1±0.3 mm in 6.1±0.3 mm in 6.1±0.3 mm in 6.1±0.3 mm in 6.1±0.3 mm in 6.1±0.3 mm in 6.1±0.3 mm in 6.1±0.3 mm in 6.1±0.3 mm in Enterobacter nterobacternterobacternterobacter nterobacternterobacternterobacter aerogenes,aerogenes,aerogenes,aerogenes, aerogenes,aerogenes,aerogenes,aerogenes, and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in and 6.4±0.4 mm in Klebsiellalebsiellalebsiella lebsiellalebsiellalebsiellalebsiellalebsiella pneumoniae. pneumoniae. pneumoniae. pneumoniae. pneumoniae. pneumoniae. pneumoniae. For all the For all the For all the For all the For all the For all the For all the For all the For all the For all the For all the For all the tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of tested microorganisms, ethanol extracts of H. maritimaH. maritimaH. maritimaH. maritimaH. maritima H. maritimaH. maritimaH. maritimaH. maritimaH. maritima showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in showed maximum antibacterial activity in E. coliE. coliE. coliE. coliE. coli E. coli. Maximum inhibMaximum inhibMaximum inhib Maximum inhibMaximum inhib Maximum inhibMaximum inhibMaximum inhib Maximum inhibMaximum inhibition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of ition zone diameter of H. maritimaH. maritimaH. maritimaH. maritimaH. maritima H. maritimaH. maritimaH. maritimaH. maritimaH. maritima were obtained in were obtained in were obtained in were obtained in were obtained in were obtained in were obtained in were obtained in were obtained in were obtained in were obtained in were obtained in were obtained in were obtained in E. cloaceaeE. cloaceaeE. cloaceaeE. cloaceaeE. cloaceaeE. cloaceae E. cloaceaeE. cloaceaeE. cloaceaeE. cloaceae and in and in and in and in E. coliE. coliE. coliE. coliE. coli E. coli with diameter with diameter with diameter with diameter with diameter with diameter with diameter with diameter with diameter with diameter with diameter with diameter 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. 6.2±0.4 mm and 6.4±0.5 mm, respectively. In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of In ethanolic extract of H. nepalensisH. nepalensisH. nepalensisH. nepalensisH. nepalensisH. nepalensis H. nepalensisH. nepalensisH. nepalensis H. nepalensisH. nepalensis, maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone , maximum inhibition zone diameter among all the tested microorganisms was obtained i diameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained i diameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained i diameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained i diameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained idiameter among all the tested microorganisms was obtained i diameter among all the tested microorganisms was obtained in E. coliE. coliE. coliE. coliE. coliE. coliE. coli DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table DD12 with diameter of 6.4±0.5 mm (Table 3). 3). H. nepalensisH. nepalensisH. nepalensisH. nepalensisH. nepalensisH. nepalensis H. nepalensisH. nepalensisH. nepalensis H. nepalensisH. nepalensis showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in showed maximum inhibition zone with diameter of 6.2±0.4 mm in E. cloaceae, E. cloaceae, E. cloaceae, E. cloaceae, E. cloaceae, E. cloaceae, E. cloaceae, E. cloaceae, E. cloaceae, E. cloaceae, E. cloaceae, E. cloaceae, 6.3±0.4 mm in 6.3±0.4 mm in 6.3±0.4 mm in 6.3±0.4 mm in 6.3±0.4 mm in 6.3±0.4 mm in 6.3±0.4 mm in 6.3±0.4 mm in 6.3±0.4 mm in E. aerogenes, aerogenes,aerogenes, aerogenes,aerogenes,aerogenes,aerogenes, and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in and 6.3±0.3 mm in K. pneumoniae. K. pneumoniae. K. pneumoniae. K. pneumoniae. K. pneumoniae. K. pneumoniae. K. pneumoniae. K. pneumoniae. K. pneumoniae. K. pneumoniae. Although the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, t Although the two stock strains are different, t Although the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, t Although the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, t Although the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, tAlthough the two stock strains are different, there was not here was not here was not here was not here was not here was not here was not here was not here was not here was not here was not here was not here was not significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences ( significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (significant differences (p > 0.05). In addition> 0.05). In addition> 0.05). In addition > 0.05). In addition > 0.05). In addition > 0.05). In addition> 0.05). In addition> 0.05). In addition > 0.05). In addition> 0.05). In addition> 0.05). In addition > 0.05). In addition> 0.05). In addition> 0.05). In addition> 0.05). In addition, for each strain,for each strain,for each strain,for each strain,for each strain,for each strain,for each strain,for each strain, for each strain,for each strain,for each strain, for each strain,for each strain, there was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four here was not significant differences among four various concentrations (various concentrations (various concentrations (various concentrations (various concentrations (various concentrations ( various concentrations (various concentrations (various concentrations (various concentrations (various concentrations (various concentrations (various concentrations (various concentrations (various concentrations ( various concentrations (various concentrations (various concentrations (various concentrations (various concentrations (various concentrations (various concentrations (p > 0.05).> 0.05).> 0.05). >

Keywords: GenusGenusGenusGenus Hydrocotyle, Paper Disk Diffusion MethodPaper Disk Diffusion MethodPaper Disk Diffusion Method Paper Disk Diffusion MethodPaper Disk Diffusion MethodPaper Disk Diffusion MethodPaper Disk Diffusion MethodPaper Disk Diffusion MethodPape