ASSOCIATION OF UBE2L3 GENE POLYMORPHISM AND INTERLEUKIN 10 GENE POLYMORPHISM WITH SYSTEMIC LUPUS ERYTHEMATOSUS AMONG WOMEN POPULATION IN CHINA

Sufiyan Pottanpulan, Song Jiquan*, Ligi Isac and Hanaasha Kumar

ABSTRACT

Objective: To investigate the association of UBE2L3 gene polymorphism and Interleukin 10 gene polymorphism with systemic lupus erythematous among woman population in china. Methods: A case－control study of 111 female SLE patients and 120 healthy individuals were designed. Genomic DNA of subjects were extracted and amplified by polymerase chain reaction (PCR). second generation/next generation sequencing technology was performed to determine the single nucleotide polymorphism(SNP) in UBE2L3(rs5754217) and IL10(rs3024505). Results: The loci of UBE2L3 rs5754217 show the frequency of genotype GT, GG, TT as follows (52.2% and 44.2%), (18.9% and 30%) and (28.8% and 25.8%) respectively in SLE case group and control group. The frequency of G and T allele as follows (45% and 55%) and (52.1% and 49.7%) respectively in SLE case group and control group. The P values, odds ratio and 95%CI of genotype GT (P=0.10, OR=1.38,95%CI= (0.824 – 2.322)) and genotype TT (P=0.30, OR=1.16,95%CI= (0.651 – 2.075)) are statically not significant(P>0.05). The P values, odds ratio and 95%CI of genotype GG (P=0.02, OR=0.54, 95%CI= (0.294 – 1.006)) are statically significant result (p<0.05). this result indicates that may be there is 0.54 times higher risk for SLE by genotype GG. The P values, odds ratio and 95%CI of allele G (P= 0.06, OR= 0.75, 95%CI = (0.522 – 1.087)) and allele T (P=0.06, OR=1.32, 95%CI = (0.919 – 1.912)) are statically not significant. Loci of IL10rs3024505 show the frequency of genotype CT, CC as follows (7.2% and 11.7%) and (92.8% and 88.3%) respectively in SLE case group and control group. The frequency of C and T allele as follows (96.4 and 94.2%) and (3.6% and 5.8%) respectively in SLE case group and control group. The P values, odds ratio and 95%CI of genotype CT (P=0.12, OR=0.58,95%CI= (0.236 – 1.460)) and genotype CC (P=0.12, OR=1.70,95%CI= (0.690– 4.224)) are statically not significant(P>0.05). The P values, odds ratio and 95%CI of allele C (P= 0.13, OR=1.65, 95%CI = (0.684 – 4.013)) and allele T (P=0.13, OR=0.60,95%CI = (0.249 – 1.461)) are statically not significant. Conclusion: The results suggest that Genotype ―GG‖ of loci rs5754217 (UBE2L3) show a positive correlation with SLE case group as compared to control group. This indicates that the genotype ―GG‖ of rs5754217 may increase the risk of SLE in Chinese women population.

Keywords: systemic lupus erythematosus (SLE); ubiquitination proteasome pathway; UBE2L3; Interleukins; IL 10; single nucleotide polymorphisms (SNPs).