IN VITRO STUDY OF ANTIMICROBIAL ACTIVITY, ANTICANCER ACTIVITY, LC/MS ANALYSIS, AND SEQUENCE OF COCONUT HUSK RETTING SOIL ASSOCIATED ACTINOMYCETES IN MANDAIKADU, KANYAKUMARI DISTRICT

Sheena A. and P. A. Mary Helen*

ABSTRACT

Actinomycetes are a source of abroad variety of secondary metabolites with diverse biological activities, such as antifungal, antibiotic. Many of which have been developed for clinical use. Actinomycetes were isolated from the coconut husk retting soil sample by serial dilution agar plating method. The antimicrobial activity of actinomycetes extract was tested against pathogenic bacteria and fungi. The extract was tested against seven bacteria and five fungi. Then bioactive compounds were purified by silica gel column chromatography and it was identified by liquid chromatography-Mass spectrometry. Cytotoxic activity of actinomycetes extract on A375 cell line were analysed by MTT method. Three compounds such as 1-3-Di undecylprodiginine, Anthracene-9,10-dione, Isorenieratine were identified. The DNA of actinomycetes was isolated and the 16S rRNA gene was amplified using universal eubacteria 16S rRNA gene primers and it was phenotypically identified as Streptomyces albaduncus.

Keywords: LC.MS, 16S rRNA, coconut husk retting soil.