OLANZAPINE COMBINED WITH ETHANOL MODULATES THE ACTIVITY OF MITOCHONDRIAL ENZYMES AND ACETYLCHOLINESTERASE IN METHYLPHENIDATE INDUCED MANIA

Thangavel Tamilselvan, Saiful Alom Siddique, Manikannan Vishnupriya and Elumalai Balamurugan*

ABSTRACT

Background/Aim: Mood disorders (unipolar and bipolar disorders) are currently the 4th leading contributor to the global burden of disease (DALYs) in 2000. The present study was aimed to evaluate the effect of olanzapine (OLZ) with ethanol (EtOH) on methylphenidate (MPD) induced mania in Swiss albino mice. Materials and Methods: The effect of EtOH on OLZ treated MPD induced manic mice was analyzed by Krebs cycle enzymes activities on isocitrate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, α-ketoglutarate dehydrogenase and acetylcholinesterase. Results: MPD induced mania was manifested by decreased the activity of Krebs cycle enzymes and AChE in brain was observed. Our result shows that OLZ controlled the activity of Krebs cycle enzymes and AChE in brain to treated MPD administrated mice. EtOH rather than being treated alone, when combined with OLZ significantly worsen the activity of Krebs cycle enzymes and AChE in MPD administrated mice. Conclusion: Based on the Krebs cycle enzymes and AChE in brain studies of our results, it can be concluded that EtOH attenuates the OLZ treatment and thereby reduced Krebs cycle enzymes and enhanced AChE activity in MPD induced manic mice. Further studies are needed to discover the mechanisms responsible for these findings.

Keywords: Manic disorder, Methylphenidate, Olanzapine, Ethanol, Krebs cycle enzymes, AChE.