EXPRESSION OF GENES (NTL, FLH, MOM AND DOC) DURING THE DEVELOPMENT OF ZEBRAFISH UNDER CHLORPYRIFOS TOXICITY

Talapati Mary Sheethal Raj, Kuder  Reshma Shabnam, Dharmapuri Gangappa and Gundala Harold Philip*

ABSTRACT

Chlorpyrifos is one of the most widely used organophosphate insecticides. It is effective against a broad spectrum of insect pests of economically important crops around the globe. This extreme usage has lead to detrimental effects on biological life. The aim of the present study was to determine the toxic effects of chlorpyrifos during early development in zebrafish. The embryo/larvae were exposed to 200μg/L and 1000μg/L of CP and observations were made at 24hpf, 48hpf and 72hpf. To understand the reasons for abnormalities four genes namely no tail (ntl), floating head (flh), momo (mom) and doc (doc) responsible for their role in development have been selected and analyzed by RT-PCR. We have shown induction in expression of all four genes. Of all the four genes induction of flh was more at 24hpf and doc was less at the same time. Comparing induction between the two treatment groups (200μg/L and 1000μg/L), higher expression was noticed at 1000μg/L for all genes except flh at 24hpf stage.

Keywords: Chlorpyrifos, Zebrafish, no tail (ntl), floating head (flh), mom (momo) and doc (doc).