SIMULTANEOUS ESTIMATION OF OFLOXACIN AND TINIDAZOLE IN SOLID DOSAGE FORM BY U V SPECTROPHOTOMETRY USING MIXED SOLVENCY CONCEPT

Sanjay Jain*, R. K. Maheshwari, Rajesh Kumar Nema and Indrajeet Singhvi

ABSTRACT

In present research work a new simple, specific, precise, accurate, robust and economical UV-Spectrophotometric method for simultaneous estimation of ofloxacin and tinidazole in tablet dosage form using mixed solvency concept was developed and validated. In the present work 10% phenol and 20% sodium benzoate blend was used as a hydrotropic solvent to increase the solubility of poorly water soluble ofloxacin and tinidazole. The analytical wavelength for ofloxacin and tinidazole are 330nm and 318 nm respectively. The developed method was validated as per ICH guidelines in terms of linearity and range, specificity, accuracy, precision and sensitivity. The percent drug estimated in tablet formulation was 95.60±0.338 for tinidazole and 97.10±0.499 for ofloxacin by method B. and similarly it was 95.26±0.504 for tinidazole and 95.33±0.594 for ofloxacin by method A respectively. The range of percent recoveries varied from 95.63±0.349 to 97.60±0.457 for tinidazole and 96.00±0.999 to 97.55±0.133 for ofloxacin. Based on the results obtained the proposed method can be regarded a simple, precise, accurate, reliable, cost effective and eco friendly for simultaneous estimation of ofloxacin and tinidazole.

Keywords: Ofloxacin, Tinidazole, UV-Spectrophotometry, solid dosage formulation, mixed solvency concept.