COMPARATIVE ANALYSIS OF ANTITHROMBOTIC EFFECT OF ETHANOL EXTRACT AND ITS DIFFERENT FRACTIONS OF STERCULIA CORDATA LEAVES

Syed Md. Abdul Kader, Md. Imdadul Haque*, Shababa Wajida Nihar, Mahmudul Hasan, Abid Newaz Shihab, Fatema Akhter, Nazia Hassan and Tajmira Zaman

ABSTRACT

Background: The purpose of the investigation was to ascertain whether the leaf extract and fractions of Sterculia cordata holds any significant antithrombotic properties. Methods: Leaves of Sterculia cordata was extracted with pure ethanol (EESC) then ethanol extract fractioned with methanol (MFEESC), n-hexane (NHFEESC) and chloroform (CHFEESC). The antithrombotic activity of the extracts and fractions were evaluated by clot lyses test using the standard streptokinase on human blood. Results: Using an in vitro antithrombotic model, all extracts, fractions, and Streptokinase exhibited significant (P < 0.0001) clot lysis. EESS showed the highest significant (62.04 ± 2.10%) clot lysis activity among all the extracts (P > 0.0001 compared to negative control and P > 0.001 compared to positive control), which is highest compared with other sample treatment. Conclusions: The overall results of the study indicated significant antithrombotic activity of different extracts and fractions of leaves of Sterculia cordata. Furthermore, this plant deserves further investigation for other paramount pharmacological activities and comprehensive research and isolation of the active constituents responsible for these activities and establishes the mechanism of action.

Keywords: Sterculia cordata, antithrombotic, extract, fraction.