IN VITRO ANTIOXIDANT, CYTOTOXIC AND IN VIVO NEUROPHARMACOLOGICAL EFFECTS OF IPOMOEA PES-TIGRIDIS

S. M. Tayabul Hossain, Abid Newaz Shihab*, Fatema Akhter, Nazia Hassan, Tajmira Zaman, Mohammad Moshiur Rahman, Rimel Barua and Mir Monir Hossain

ABSTRACT

Background: Ipomoea pes-tigridis (Tiger’s foot) is an important plant grown in Bangladesh. The local name of tiger’s foot is Languli lala. Different parts of this plant used in the treatment of tumors and cancers. Leaf powder is smoked to get relief from bronchial spasm. Ipomoea pes-tigridis has been investigated for in vitro antioxidant, cytotoxic and in vivo neuropharmacological activities. The phytochemical constituents of the dried powdered leaves were extracted using Petroleum Benzine. Methods: Primary phytochemical screening was accomplished by using established methods. Antioxidant effect was conducted with DPPH method and Cytotoxicity was studied by brine shrimp lethality test. The in vivo action was done using mice of both sexes. Neuropharmacological activity evaluated by open field and hole cross tests. Results: Alkaloids, Flavonoids, Glycosidase, Saponins and Carbohydrate groups were present in the extract. The extract showed a dose dependent radical scavenging effect in DPPH assay. IC50 for free radicals achieved by the extract is 79.05 μg/ml. Leaf Petroleum Benzine extract of Ipomoea pes-tigridis showed lethality in a dose dependent manner. LC50 value of leaf extract of Ipomoea pes-tigridis was found 12.035 μg/ml. The extract delivered critical sedative impact at the doses of 200 mg/kg and 400 mg/kg (by oral route) treated with reference substance diazepam (DZP) observed in case of mice at both open field and hole cross test. Conclusion: The present research suggests that Petroleum Benzine extract of Ipomoea pes-tigridis has significant antioxidant, cytotoxic and neuropharmacological activities. Based on the findings of antioxidant, cytotoxic and neuropharmacological activities, we can say that the obtained results support for the uses of this plant as traditional medicine.

Keywords: Ipomoea pes-tigridis, Petroleum Benzine, antioxidant, cytotoxic, neuropharmacological.