TOXICOLOGICAL AND PHYTOCHIMICAL SCREENING STUDY OF CRINUM SCILIFOLIUM, PLANT OF COTE D’IVOIRE

Koffi Francis Bienvenu, Miezan Bilé Aka Patrice, Okpékon Timothée and Yapi Houphouet Félix*

ABSTRACT

The leaves of Crinum scilifolium, are used in traditional medicin to treat epilepsy. The present study was carried out to screen aqueous and ethanolic extract of this plant for its phytochemical constituents and acute toxicity profile. Acute toxicity study was evalauted in rodent by OCDE guideline 423 and phytochemical analysis was performed according standard methods. Rats were orally treated by 50, 300, 500, 2000, and 5000 mg/kg body weight with both extracts ony once. They were observed for 24H, with special attention given to the first 4H and once daily further for period of 14 day. Phytochemical components were identified in the plants extracts by using standart laboratory grade reagents. The results revealed the presence of sterols, flavonoids, polyphenols and alkaloids. The aqueous one countains the same compounds and saponins. The results of acute toxicity study classified Crinum scilifolium above the hazard category 500 mg/Kg < DL50 < 2000 mg/Kg according to global harmonized classification system. The results obtained suggest that the plant extract is considered as slightly toxic. The diversity of phytochemicals found suggest that Crinum scilifolium leaves could serve as a source of useful drugs.

Keywords: acute toxicity, Crinum Scilifolium, phytochemistry, Côte d’Ivoire.