ANTIMICROBIAL ACTIVITIES OF MOUNTAIN NEEM (MELIA AZEDARACH) IN THE AREAS OF WOLLEGA UNIVERSITY, NEKEMTE, ETHIOPIA.

S. Vijayalakshmi* and G. Theenadhayalan

ABSTRACT

Mountain Neem (Melia azedarach) trees are found in the campus of Wollega University as ornamental plant. This study used to find out the medicinal properties of Melia azedarach. It used as home medicine in India and it may contains more bioactive compounds to treat various diseases such as Diabetes, Skin diseases and some plant diseases, etc., In this study the crude extracts of Mountain neem examined to determine the antimicrobial activities using Disc diffusion assay method against Five bacterial and TLC bioautography against two fungal pathogens(Bacterial pathogens: Salmonella typhi, Bacillus subtilis, Pseudomonas aeruginosa, E.coli, and Staphylococcus aureus. Fungal pathogens: Candida sp., Aspergillus sp., and Botrytis ceneria). The Ethyl acetate extract of Melia azedarach exhibited broad spectral activity against both bacterial and fungal pathogens. Methonolic extracts exhibited highest activity on Aspergillus niger, Candida albicans and Bacillus subtilis. Medium activity by aqueous extract on E.coli. Therefore the crude extracts of Mountain neem exhibited Antibacterial and antifungal activities. The active compound isolation and identification is necessary to find out the pharmacological important compounds to treat various diseases.

Keywords: Anti microbial activity, Mountain neem, bioactivity, Anti diabetics and Medicinal plant.