EVALUATION OF ANTIDIABETIC ACTIVITY OF ETHANOLIC EXTRACTION OF LEAVES OF RHINACANTHUS NASUTUS (L.)

Suresh V.*, Senthilkumar N., Ganesh Kumar T., Yuva Srinivas G., Varadharajan V., Tamilselvan A., Vasu Devan P.

ABSTRACT

Objective: To evaluate the antidiabetic effects of ethanolic extracts of Rhinacanthus nasutus leaves (ERN) in Wister rats. Methods: Alloxan induced diabetic test model were performed to evaluate antidiabetic activity of ERN at two different doses 200 and 400 mg/kg respectively. Ethanolic extract of Rhinacanthus nasutus leaves (Acanthaceae) was tested for anti-diabetic activity for alloxan induced diabetics in wistar rats. After oral administration of the extract at two different doses (200 and 400mg/kg body weight) for 21 days to alloxan-induced diabetic rats, the blood glucose, level was assayed periodically on 0, 7, 14 and 21th day. After 21 days treatment all biochemical parameters like total cholesterol, triglyceride, total protein levels were checked and compare with control and standard group and body weight was also determine which was compare with initial weight. Results: Ethanolic extract shown significant protection and lowered induced blood glucose level to normal in glucose tolerance test on the day 0,7, 14 and 21th compare with diabetic control and standard groups. There was significant control of all biochemical parameters levels like total cholesterol, triglyceride, total protein in extracts of Rhinacanthus nasutus leaves treated diabetic rats. Marked body weight loss was observed in diabetic rats. The data obtained from this study showed that the treatment of extracts Rhinacanthus nasutus leaves protect the diabetic rats from loss of massive body. Conclusions: These results indicate that the Rhinacanthus nasutus leaves posses significant anti-diabetic activity.

Keywords: Antidiabetic activity, Rhinacanthus nasutus, Acanthaceae, Alloxan- induced diabetis, biochemical parameters.