COMPARATIVE STUDY OF TWO CLASSICAL EXTRACTION METHODS BY USING GC/MS OF TEPHROSIA NUBICA BOISS GROWING IN SAUDI ARABIA

Hanan Al-Yousef*, Ghada Anber, Nawal Al-Qahs and Al-Anous Bin Swedan

ABSTRACT

A comparative study was done at first time from the aerial parts of T. nubica Boiss growing in S. A. by using two conventional extraction techniques Macerated (MA) & Soxhlet (SX) and analyzed by GC & GC/MS. In this work, we compare the extraction effectiveness of two methods commonly applied in lab. Also, a comparison of extracting solvents was performed in current study. The extraction conditions might not be maximized for these two techniques; the select of extraction parameters were depend on the experience from pervious investigation published worldwide. This study focused on the vast variation of the extraction quantities and quality with MA & SX methods. The GC & GC/MS chromatographic analysis results found have observed that the alcohol can give the highest recoveries when compared to other extraction solvents. The comparison made between the MA and SX extraction methods has found that MA is a suitable alternative to SE for the hexane and chloroform chemical composition, but SX did not give a good recovery by these two solvents. This study is the first report describing comparison between MA and SX by using GC & GC/MS.

Keywords: T.nubica, SX, Maceration, GC&GC/MS.