DETERMINATION AND QUANTIFICATION OF AVAROL IN DYSIDIA AVARA BY HPLC-DAD

Özge DEMIR, Hajar Heydari, Bülent Gozcelioglu and Belma Konuklugil*

ABSTRACT

Avarol is major compound of Dysidia avara. In this study avarol has been quantified by using reverse-phase High Performance Liquid Chromatography (HPLC) with diode array detector (DAD). The identification of avarol has been based on retention time and UV spectra by comparison with standard. The experimental results have shown high amount of avarol in D. avara from Ayvalık Turkey. The precision, accuracy, and reproducibility of the method have been apparent from good linearity.

Keywords: Avarol, Dysidia avara, HPLC-DAD, Quantification.