IN-VITRO CHANGES IN THE LIVER FUNCTION TEST OF SICKLE CELL ANEMIA PATIENTS BY AZOCOMPOUNDS

Pallavi Mehere, Virendra G. Meshram* and B. A. Mehere

ABSTRACT

The most common and best-known type of sickle cell disease is sickle cell anemia, which is also called SS disease. SCA is an inherited blood disorder caused by single nucleotide substitution in the β-globin gene that renders their hemoglobin (HbS) much less soluble than normal hemoglobin (HbA) when deoxygenated. The polymerization of HbS upon deoxygenation is the basic pathophysiologic event leading to RBC sickling, hemolysis, vasoocclusion and ultimately to chronic organ damage. The aim of the study was to evaluate in-vitro the changes in the biochemical parameters of sickle cell anemia patients by Azocompounds. The study was conducted with 25 sickle cell anemia patient’s blood samples form Nagpur area. Azocompounds like Hydroxyurea (HU) has been reported to be effective in improving survival and reducing morbidity in some SCA patients. Inspired by the effectiveness of this drug, the further study focuses on the testing of other Azocompounds on the sickle RBCs in this disorder. The p-amino benzoic acid and its derivatives poses a wide variety of pharmacological properties such as anti-bacterial, anti-fungal, anti-tuberculosis, analgesic, local anesthetic. Serial liver function tests were performed in each case. The significance of the tests in this disease is discussed and the importance of serial determinations is indicated. Elevated level of Total bilirubin level was significantly decreased by the Azocompounds. The preliminary results in the biochemical parameters are noticeable and encourage further investigations.

Keywords: Sickle cell disease, Sickle cell anemia, hemoglobin S, Azocompounds, Hydroxyurea.