BARLERIA CRISTATA: A COMPARATIVE ANALYSIS OF PHYTOCHEMICAL, CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF LEAF AND BARK EXTRACTS

Mukul Pathy, Tanvi Sharma and Sunita Bhatnagar*

ABSTRACT

Phytochemical, cytotoxic and antioxidant profiles of leaf and bark of the ornamental plant Barleria cristata was explored in the present study. Hexane, Chloroform, Acetone and Methanol solvents were prepared using successive extraction method. All the extracts were tested for their cytotoxic activity using Brine shrimp lethality assay. Antioxidant activity of the extract was assessed using qualitative and quantitative assays. For qualitative, Thin layer chromatography based DPPH assay and for quantitative, Nitric oxide radical scavenging, DPPH radical scavenging and Ferric reducing antioxidant power (FRAP) were conducted. Leaf and bark extracts were found to contain terpenoids, anthraquinones, alkaloids, flavonoids and saponins. Methanol extract of Barleria cristata leaf showed potent bioactivity/cytotoxic and antioxidant activity.

Keywords: Cytotoxic, brine shrimp, antioxidant, DPPH.