A COMPARISON OF PENETRATION OF SODIUM HYPOCHLORITE LABELED WITH ALIZARIN RED FLUORESCENT DYE INTO DENTINAL TUBULES OF ROOT CANAL SYSTEM USING THREE DIFFERENT TECHNIQUES OF IRRIGATION: AN IN VITRO FLUORESCENT MICROSCOPE STUDY

Dr. Ajay Singh Rao, Dr. Anand Mangalgi, *Dr. Kundan Shah, Dr. Syed Furqhanuddin, Dr. Arif Mohammad Maseehuddin and Dr. Deepika mallasure

ABSTRACT

Aim and objectives: To compare the effect of three different techniques of irrigation on penetration of Sodium hypochlorite into dentinal tubules of root canal system using alizarin red fluorescent dye labeled with sodium hypochlorite. Materials and Methods: Forty eight extracted maxillary anterior teeth were instrumented up to Pro-taper finishing file F5. They were randomly divided into four groups (N = 12). Group 1 was the control group, rest all the groups (Group 2, 3, & 4) were experimental groups. After removing the smear layer using EDTA Gel (Glyde, Dentsply) followed by 5ml of normal saline, A final rinse of 5 ml of 3% NaOCl labeled with a fluorescent dye 0.2% alizarin red was done using syringe irrigation with a 24-Gauge syringe in Group 2, Intermittent passive ultrasonic irrigation (IPUI) in Group 3 and Continuous passive ultrasonic irrigation (CPUI) in Group 4. Control Group (Group 1) had a final rinse of 5 ml of plane 3% NaOCl sodium hypochlorite i.e.; not labeled with 0.2% alizarin red dye using a 24-Gauge syringe. The Specimens were sectioned at 1, 3 & 5 mm from the apex in 1-mm thick sections, ground and prepared for fluorescence microscopy. NaOCl penetration into dentinal tubules was analyzed using Student t- test analysis. Results: At 1 mm from the apex, the penetration of NaOCl was significantly higher (p<0.05) for Group 4 (CPUI group). Conclusion: CPUI appears to be more effective than IUPI and syringe irrigation techniques.

Keywords: passive ultrasonic irrigation, alizarin red, fluorescent microscope, sodium hypochlorite.