EFFECT OF LEPTADENIA HASTATA HEXANE LEAF EXTRACTS AGAINST HEAMOTOLOGICAL, BIOCHEMICAL AND INDOMETHACIN INDUCED ULCER IN RATS

Isaac John Umaru*, Fasihuddin A. Badruddin and Zaini B. Assim

ABSTRACT

Objectives: This study was targeted at valuing a claim by traditional herbal practitioner that the leaves of Leptadenia hastata possess ulcer healing property by assessing the effect of Leptadenia hastata on ulcer induced rats. Material and method: The effects of an hexane leaf extracts of Leptadenia hastata were studied in 40 white albino rats over a period of 21days, to ascertain the claim of Leptadenia hastata has ulcerogenic properties, the rats were divided into eight groups those in group one served as control group, group two negative control (Indomethacin) ulcer induced with no treatment, while group three positive control (Omeprazole) and groups four to eight are dosed groups ranged from; 100mg/kg 200mg/kg, 300mg/kg, 400mg/kg and 500mg/kg extracts respectively, Microscopic examination was carried out and scored for the presence of lesion. The length and breadth of the lesion of the stomach was measured for ulcer index. Stomach and Blood sample were collected for Histological, hematological and biochemical analysis. The specimen of the stomach was taken for histopathological studies. Results: The study showed that the extracts of Leptadenia hastata caused increased in the weight of the rats compared to the negative control and the levels of packed cell volume, hemoglobin concentration, red blood cell, white blood cell, mean corpuscular volume and mean corpuscular hemoglobin. The changes in the biochemical parameter were all within the range of the control. Histologically, stomach degeneration was characterized by lesion and decrease number of lining cell of the epithelium. Conclusion: The study indicate that hexane leaves crude extracts of Leptadenia hastata possess ulcer healing activity.

Keywords: Leptadenia hastata, ulcer, rats, histopathology, haematology, serum biochemistry.