ISOLATION AND MOLECULAR IDENTIFICATION OF PATHOGENIC BACTERIA AND FUNGI FROM CLINICAL SAMPLES: COMPARATIVE INHIBITION BY ANTIBIOTICS

Alaa Sameer Neamah*

ABSTRACT

Hospital environment is the major cause of various infections including cut and burn wounds. Burn wounds are one of the most common and devastating forms of trauma, exposing the immune suppressed patients to early and serious infections. Faster detection of the causative Microbes and institution of proper therapy would help greatly in preventing septic complications. Recent advances in the field of Molecular Biology, including the amplification of genetic material by the Polymerase Chain Reaction (PCR) technologies have led to faster and more reliable microbial detection methods. Results would help in early detection of causative organisms and treatment regimes in patients with burn wounds.

Keywords: Clinical Samples, PCR, Antibiotics, Klebesiella.