A STUDY ON ISOLATION AND PURIFICATION OF EUGENOL FROM CLOVE BY COLUMN CHROMATOGRAPHY

Mamatha M.*, Chaluvaraju K. C., Shantharam U., Ranjeetha M., Darshitha R. and Varsha C.

ABSTRACT

In the present study the phytomedicine Eugenol is extracted from Clove in order to prepare its semisynthetic derivaties bearing 1,2,4-triazole motiff. The clove oil was subjected to isolation of Eugenol by column chromatography. The stationary phase used is silicagel (230-400 mesh size) and the mobile phase was nhexane: ethylacetate in the ratio of 7:3. About six fractions were collected from the elution. On TLC studies third and fourth fraction collected were found to contain Eugenol on comparision with standard Eugenol. Further these eluents were combined and evaporated to get yellowish oil of pure Eugenol. This isolated oil was characterised by IR, 1H NMR and GC-MS studies. The results of these characterisation studies were in good aggrement with the structural features of Eugenol.

Keywords: Eugenol, Isolation, Column Chromatography, 1H NMR, GC-MS.