ISOLATION OF NEW SAPONINS FROM BELLIUMBELLIDIOIDES L. FAMILY ASTERACEAE

MHD Isam Hasan Agha*

ABSTRACT

Belliumbellidioides is a small herbal plant (9-15 cm). The origin of this plant is Corsica. Due to the presence of high concentration of Saponins in this plant, Bellium bellidioideswas the aim of this study. Saponins have special structural features, therefore extraction and isolation of saponins poses a serious challenge. However, in general it is difficult to use a single technique for isolation of saponins, but using Column chromatography on Sephadex 20 and on Silica gel (0.2-0.063 mm) in addition to HPLC on RP18 it could be isolated about 20 –30 and 50 mg of Saponins Agha BS2, AghaBS3 and AghaBS4 in a semi pure form. Using H1NMR and C13NMR Spectrometry in addition to acid hydrolysation and TLC methods it could be determined that the Aglycone is Polygalic acid and the sugars are Rhamnose and glucose.

Keywords: Saponins, extraction, HPLC, H1-NMR, C13-NMR.